B-hROR1 CT26.WT_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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B-hROR1 CT26.WT

Common name	B-hROR1 CT26.WT	Catalog number	322263
Aliases	NTRKR1, dJ537F10.1	Disease	Colon carcinoma
Organism	Mouse	Strain	BALB/c
Tissue types	Colon	Tissue	Colon

Description

The mouse Ror1 gene was replaced by human ROR1 coding sequence in B-hROR1 CT26.WT cells. Human ROR1 is highly expressed on the surface of B-hROR1 CT26.WT cells.

Application

B-hROR1 CT26.WT cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hROR1 CT26.WT cells. The exogenous promoter and human ROR1 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Ror1 gene, resulting in a non-functional transcript.

Protein expression analysis

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ROR1 expression analysis in B-hROR1 CT26.WT cells by flow cytometry. Single cell suspensions from wild-type CT26.WT and B-hROR1 CT26.WT cultures were stained with species-specific anti-ROR1 antibody. Human ROR1 was detected on the surface of B-hROR1 CT26.WT cells but not wild-type CT26.WT cells.