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B-hFGL1 MC38
Common name
B-hFGL1 MC38 Catalog number     311270
Aliases HFREP1, HP-041, HPS, LFIRE1,
  fibrinogen like 1
Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse Fgl1 gene was replaced by human FGL1 coding sequence in B-hFGL1 MC38 cells. Human FGL1 is highly expressed and secreted by B-hFGL1 MC38 cells.


Application

B-hFGL1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hFGL1 MC38 cells. The exogenous promoter and human FGL1 coding sequence was inserted to replace part of murine exon 4 and all of exons 5-7. The insertion disrupts the endogenous murine Fgl1 gene, resulting in a non-functional transcript.

Protein expression analysis


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FGL1 expression analysis in B-hFGL1 MC38 cells by ELISA. Supernatant from wild-type MC38 and B-hFGL1 MC38 cultures were stained with species-specific anti-FGL1 antibody. Human FGL1 was detectable in the supernatant of B-hFGL1 MC38 cells but not in that of wild-type MC38 cells. The 3-A07 clone of B-hFGL1 MC38 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hFGL1 MC38 cells. B-hFGL1 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hFGL1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.