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B-hB7-H3 EL4
Common name
B-hB7-H3 EL4 Catalog number    310705
Aliases 4Ig-B7-H3, CD276, B7RP-2 Disease  Lymphoma
Organism
Mouse Strain  C57BL/6N
Tissue types Lymphoma Tissue  T lymphocyte

Description


The mouse B7h3 gene was replaced by human B7H3 coding sequence in B-hB7-H3 EL4 cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 EL4 cells.


Application


B-hB7-H3 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


Gene targeting strategy for B-hB7-H3 EL4 cells. The exogenous promoter and human B7H3 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine B7h3 gene, resulting in a non-functional transcript.


Protein expression analysis




B7-H3 expression analysis in B-hB7-H3 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hB7-H3 EL4 cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 EL4 cells but not wild-type EL4 cells. The 1-H02 clone of B-hB7-H3 EL4 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes




Subcutaneous homograft tumor growth of B-hB7-H3 EL4 cells. B-hB7-H3 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6N mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.Animals with tumor volume over 3000mm3 in the control group were euthanized.


Protein expression analysis of tumor cells


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B-hB7-H3 EL4  cells were subcutaneously transplanted into C57BL/6N mice (n=5), and on 27 days post inoculation, tumor cells were harvested and assessed for human B7-H3 expression by flow cytometry. As shown, human B7-H3 was highly expressed on the surface of tumor cells. Therefore, B-hB7-H3 EL4  cells can be used for in vivo efficacy studies of novel B7-H3 therapeutics.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hB7-H3 EL4 cells. B-hB7-H3 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.