||B-hB7-H3 EL4||Catalog number||310705|
|Aliases||4Ig-B7-H3, CD276, B7RP-2||Disease||Lymphoma|
|Tissue types||Lymphoma||Tissue||T lymphocyte|
The mouse B7h3 gene was replaced by human B7H3 coding sequence in B-hB7-H3 EL4 cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 EL4 cells.
B-hB7-H3 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Gene targeting strategy for B-hB7-H3 EL4 cells. The exogenous promoter and human B7H3 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine B7h3 gene, resulting in a non-functional transcript.
Protein expression analysis
Subcutaneous homograft tumor growth of B-hB7-H3 EL4 cells. B-hB7-H3 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6N mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.Animals with tumor volume over 3000mm3 in the control group were euthanized.
Protein expression analysis of tumor cells