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B-Camk2a-iCre-EGFP mice
Strain Name

C57BL/6-Camk2atm1(icre-EGFP)/Bcgen

Stock No.  110780
Common name

B-Camk2a-iCre-EGFP mice

Gene symbol and name  Camk2a, calcium/calmodulin-dependent protein kinase II alpha
Strain of origin

C57BL/6

Chromosome  18
Coat color Black Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
12322

Application

  • Function research of genes
  • Function research of synaptic plasticity and Long Term Potentiation (LTP) in hippocampal networks

Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

the forebrain, including the CA1 pyramidal cell layer of the hippocampus


Gene editing strategy


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A targeting vector was designed to insert an P2A-iCre-P2A-EGFP cassette before the calcium/calmodulin-dependent protein kinase II alpha (Camk2a) stop codon. A Frt-flanked neomycin resistance (Neo) gene was inserted into the intron16-17 of Camk2a gene. The targeting vector was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to FLP transgene mice to delete the neomycin selection cassette. FLP transgene was removed by crossing with wild-type C57BL/6 mice. These mice were maintained on a C57BL/6 background.


Phenotype Analysis


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The flow results showed that the mouse brain could express ICRE and function normally.

The single positive cells of TDTomato were found in testis, heart, liver, spleen, lung and kidney by flow cytometry.The experiment results with Jackson Lab B6. Cg - Tg (Camk2a - cre) STL T29-1 / J (Stock No: 005359 | T29-1) multiple organ test in mice to express the result of the agreement.The IF results of the fluorescent microscopic imaging of the mouse brain showed that the red fluorescent protein of TDTomato was successfully expressed in the mouse brain, and the expression was significantly increased in some areas, especially in the structure of the dentate gyrus, which was highly expressed.


reference


1. Mayford M, Bach ME, Huang YY, Wang L, Hawkins RD, Kandel ER. Control of memory formation through regulated expression of a CaMKII transgene. Science. 1996 Dec 6;274(5293):1678-83. [PMID: 8939850]

2. Kellendonk C, Tronche F, Casanova E, Anlag K, Opherk C, Schütz G. Inducible site-specific recombination in the brain. J Mol Biol. 1999 Jan 8;285(1):175-82. [PMID: 9878397]

3. Mantamadiotis T, Lemberger T, Bleckmann SC, Kern H, Kretz O, Martin Villalba A, Tronche F, Kellendonk C, Gau D, Kapfhammer J, Otto C, Schmid W, Schütz G. Disruption of CREB function in brain leads to neurodegeneration. Nat Genet. 2002 May;31(1):47-54. [PMID: 11967539]

4. Minichiello L, Korte M, Wolfer D, Kühn R, Unsicker K, Cestari V, Rossi-Arnaud C, Lipp HP, Bonhoeffer T, Klein R. Essential role for TrkB receptors in hippocampus-mediated learning. Neuron. 1999 Oct;24(2):401-14. [PMID: 10571233]

5. Saito H, Yoshida T, Yamazaki H, Suzuki N. Conditional N-rasG12V expression promotes manifestations of neurofibromatosis in a mouse model. Oncogene. 2007 Jul 12;26(32):4714-9. [PMID: 17237809]

6. Küry S, van Woerden GM, Besnard T. De Novo Mutations in Protein Kinase Genes CAMK2A and CAMK2B Cause Intellectual Disability. Am J Hum Genet. 2017 Nov 2;101(5):768-788. [PMCID: PMC5673671]