Protein expression analysis

Strain specific MSLN protein expression analysis in wild-type mice and B-hMSLN mice by western blotting. Mouse MSLN was detectable in heterozygous B-hMSLN mice (H/+) and wild-type mice (+/+). Human MSLN was detectable only in B-hMSLN mice (H/+).

MSLN expression pattern analysis by IHC

Representative human MSLN expression in different tissues of B-hMSLN mice by IHC. Tissues were stained with antibodies specific for human MSLN (A-H) or anti-IgG antibody (I). Bladder, kidney, liver, heart, lung and testis of B-hMSLN mice show human MSLN positive. Human lung carcinoma as a positive control (G); Dog brain was a negative control (H); Mouse lung tissues from the wild-type C57BL/6 mice (I). Other tissues were got from homozygous B-hMSLN mice (A-F). Original magnification ×200. Abbreviations: IHC, immunohistochemistry.

MSLN expression analysis by IHC

Blood chemistry of B-hMSLN mice