Western blot analysis of FGFRL1 protein expression in homozygous B-hFGFRL1 mice. Various tissue lysates were collected from C57BL/6 wildtype mice (+/+) and homozygous B-hFGFRL1 mice (H/H), and then analyzed by western blot with species-specific anti-FGFRL1 antibody. 40 μg total proteins were loaded for western blotting analysis. FGFRL1 was detected in lung.

Strain specific analysis of FGFRL1 mRNA expression in wild-type C57BL/6 mice and B-hFGFRL1 mice by RT-PCR. Kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hFGFRL1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FGFRL1 primers. Mouse Fgfrl1 mRNA was detectable only in wild-type C57BL/6 mice. Human FGFRL1 mRNA was detectable only in homozygous B-hFGFRL1 mice but not in wild-type mice.