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B-hADA mice
Strain Name C57BL/6-Adatm1(ADA)Bcgen/Bcgen Common Name  B-hADA mice
Background C57BL/6 Catalog number 111898
Aliases 

ADA1

Protein expression analysis

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Strain specific ADA expression analysis in homozygous B-hADA mice by western blot. Tissues were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADA mice (H/H), and analyzed by western blot with anti-ADA antibody. ADA was detectable in homozygous B-hADA mice.

mRNA expression analysis

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Strain specific analysis of ADA mRNA expression in wild-type C57BL/6 mice and B-hADA mice by RT-PCR. Spleen RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADA mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADA primers. Mouse Ada mRNA was detectable in wild-type C57BL/6 mice. Human ADA mRNA was detectable only in homozygous B-hADA mice but not in wild-type mice.