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B-hPD-L1/hOX40 mice
Strain Name C57BL/6-Cd274tm1(CD274)Tnfrsf4tm1(TNFRSF4)/Bcgen Common Name  B-hPD-L1/hOX40 mice
Background C57BL/6 Catalog number  120534
Related Genes 
CD274 (CD274 antigen)
TNFRSF4
(tumor necrosis factor receptor superfamily, member 4)
NCBI Gene ID
60533,22163

Protein expression analysis




Strain specific OX40 and PD-L1 expression analysis in homozygous B-hPD-L1/hOX40 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hOX40 (H/H) mice stimulated with anti-CD3ε in vivo (7.5 μg/mice), and analyzed by flow cytometry with species-specific anti-OX40 antibody. Mouse OX40 and PD-L1 were exclusively detected in WT mice. Human OX40 and PD-L1 were exclusively detected in homozygous B-hPD-L1/hOX40 but not WT mice.


Combination therapy of PD-L1 Ab and OX40 Ab



Antitumor activity of anti-hOX40 antibody combined with anti-hPD-L1 antibody in B-hPD-L1/hOX40 mice. (A) Anti-hOX40 antibody combined with anti-hPD-L1 antibodies inhibited MC38-hPD-L1 tumor growth in B-hPD-L1/hOX40 mice. Murine colon cancer MC38-hPD-L1 cells (5×105) were subcutaneously implanted into homozygous B-hPD-L1/hOX40 mice (female, 6-7 week-old, n=6). Mice were grouped when tumor volume reached approximately 150±50 mm3, at which time they were treated with anti-hOX40 antibody combined with anti-hPD-L1 antibody with doses and schedules indicated in panel (B) Body weight changes during treatment. As shown in panel A, combination of anti-hOX40 and anti-hPD-L1 antibody shows more inhibitory effects than individual groups, demonstrating that the B-hPD-L1/hOX40 mice provide a powerful preclinical model for in vivo evaluating combination therapy efficacy of hOX40 antibodies and hPD-L1 antibodies. Values are expressed as mean ± SEM.