top
Please input keywords
Order
*Country
日本
アメリカ
中国
オーストラリア
シンガポール
イギリス
フランス
ドイツ
スイス
イタリア
カナダ
韓国
オランダ
ベルギー
スウェーデン
その他
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hCD16A mice
Strain Name C57BL/6-Fcgr4tm2(FCGR3A)/Bcgen  Common Name  B-hCD16A mice
Background C57BL/6 Catalog number  111173
Related Genes 
FCGR3A (Fc fragment of IgG receptor IIIa), CD16A

mRNA expression analysis


from clipboard



Strain specific analysis of CD16A gene expression in wild-type mice and homozygous B-hCD16A mice by RT-PCR. 
Mouse Fcgr4 mRNA was exclusively detectable in wild-type mice and human FCGR3A mRNA was exclusively detectable in homozygous B-hCD16A mice (H/H).


Protein expression analysis in NK cells



from clipboard


Strain specific CD16A expression analysis in wild-type C57BL/6 mice and homozygous B-hCD16A mice by flow cytometry. 
Splenocytes were collected from wild-type C57BL/6 mice(+/+)  and homozygous B-hCD16A mice (H/H). Human CD16A was exclusively detectable in the NK cells of homozygous B-hCD16A mice.


Protein expression analysis in macrophages

from clipboard



Strain specific CD16A expression analysis in wild-type C57BL/6 mice and homozygous B-hCD16A mice by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type C57BL/6 mice(+/+)  and homozygous B-hCD16A mice(H/H). Mouse CD16A was exclusively detectable in wild-type C57BL/6 mice. Human CD16A was exclusively detectable in homozygous B-hCD16A mice.


Protein expression analysis in multiple lymphocyte subtypes
NK cells

from clipboard


Strain specific CD16A expression analysis in wild-type C57BL/6 mice and homozygous B-hCD16A mice by flow cytometry. 
Different tissues were collected from wild-type C57BL/6 mice(+/+)  and homozygous B-hCD16A mice (H/H). Human CD16A was exclusively detectable in the NK cells of homozygous B-hCD16A mice.


Granulocytes

from clipboard



Strain specific CD16A expression analysis in wild-type C57BL/6 mice and homozygous B-hCD16A mice by flow cytometry. 
Different tissues were collected from wild-type C57BL/6 mice(+/+)  and homozygous B-hCD16A mice (H/H). Mouse CD16A was exclusively detective in granulocytes of wild-type mice. Human CD16A was not detectable in granulocytes of wild-type C57BL/6 mice or homozygous B-hCD16A mice.


Macrophages

from clipboard



Strain specific CD16A expression analysis in wild-type C57BL/6 mice and homozygous B-hCD16A mice by flow cytometry. Different tissues were collected from wild-type C57BL/6 mice(+/+)  and homozygous B-hCD16A mice (H/H). Mouse CD16A was exclusively detective in macrophages of wild-type mice. Human CD16A was exclusively detectable in macrophages of homozygous B-hCD16A mice .


Summary

from clipboard


Protein expression analysis in NK cells


from clipboard



Strain specific CD16A expression level analysis in NK cells of homozygous B-hCD16A mice and human PBMCs by flow cytometry.  


Analysis of leukocytes subpopulation in B-hCD16A  mice

from clipboard



Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hCD16A mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.


Analysis of spleen T cell subpopulations in B-hCD16A  mice

from clipboard



Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hCD16A mice (n=3, 6-week-old).  Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.


Analysis of leukocytes subpopulation in B-hCD16A  mice

from clipboard



Analysis of LNs leukocyte subpopulations by FACS. LNs were isolated from female C57BL/6 and B-hCD16A mice (n=3, 6-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.


Analysis of LNs T cell subpopulations in B-hCD16A  mice

from clipboard



Analysis of LNs T cell subpopulations by FACS. LNs were isolated from female C57BL/6 and B-hCD16A mice (n=3, 6-week-old).  Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.


Analysis of leukocytes subpopulation in B-hCD16A  mice

from clipboard



Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female C57BL/6 and B-hCD16A mice (n=3, 6-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.


Analysis of blood T cell subpopulations in B-hCD16A mice

from clipboard



Analysis of blood T cell subpopulations by FACS. Blood were isolated from female C57BL/6 and B-hCD16A mice (n=3, 6-week-old).  Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.