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B-hAXL mice
Strain Name C57BL/6-Axltm1(AXL)/Bcgen Common Name  B-hAXL mice
Background C57BL/6 Catalog number  110908
Related Genes 
AXL (ARK; UFO; JTK11; Tyro7)

Model description


Axl encodes a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The TAM receptors (Tyro3, Axl and MerTK) are promising therapeutic targets on tumor-associated macrophages. The TAM receptors are a family of receptor tyrosine kinases with shared ligands Gas6 and Protein S that skew macrophage polarization towards a pro-tumor M2-like phenotype. In macrophages, the TAM receptors also promote apoptotic cell clearance, a tumor-promoting process called efferocytosis. The TAM receptors bind the "eat-me" signal phosphatidylserine on apoptotic cell membranes using Gas6 and Protein S as bridging ligands. The Gas6/AXL signalling pathway is associated with tumour cell growth, metastasis, invasion, epithelial-mesenchymal transition (EMT), angiogenesis, drug resistance, immune regulation and stem cell maintenance. Different therapeutic agents targeting AXL have been developed, typically including small molecule inhibitors, monoclonal antibodies (mAbs), nucleotide aptamers, soluble receptors, and several natural compounds. 



Protein expression analysis


from clipboard




Strain specific AXL expression analysis in heterozygous B-hAXL mice by flow cytometry. Leukocytes from liver, spleen, lung and kidney were collected from heterozygous B-hAXL mice (H/+) respectively, and analyzed by flow cytometry with human-specific anti-AXL antibody. Human AXL was detectable in heterozygous B-hAXL mice.

Protein expression analysis

from clipboard




Strain specific AXL expression analysis in heterozygous B-hAXL mice by flow cytometry. Leukocytes from liver, spleen, lung and kidney were collected from heterozygous B-hAXL mice (H/+) respectively, and analyzed by flow cytometry with human-specific anti-AXL antibody. Human AXL was detectable in heterozygous B-hAXL mice.

References

【1】Gay CM, Balaji K, Byers LA. Giving AXL the axe: targeting AXL in human malignancy. Br J Cancer. 2017;116(4):415-423. doi:10.1038/bjc.2016.428
【2】Zhu C, Wei Y, Wei X. AXL receptor tyrosine kinase as a promising anti-cancer approach: functions, molecular mechanisms and clinical applications. Mol Cancer. 2019;18(1):153. Published 2019 Nov 4. doi:10.1186/s12943-019-1090-3
【3】Tanaka M, Siemann DW. Gas6/Axl Signaling Pathway in the Tumor Immune Microenvironment. Cancers (Basel). 2020;12(7):1850. Published 2020 Jul 9. doi:10.3390/cancers12071850