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B-Tg(hIFNG/hIL26) mice
Strain Name
C57BL/6N-Tg(hIFNG/hIL26)Bcgen/Bcgen
Common Name  B-Tg(hIFNG/hIL26) mice
Background C57BL/6N Catalog number 131596
Aliases  IFNG: IFG, IFI, IMD69,IFN-γ
IL26: AK155, IL-26
Protein expression analysis

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Strain specific IFNγ expression analysis in transgenic B-Tg(hIFNG/hIL26) mice by ELISA. Serum were collected from wild-type mice C57BL/6 mice (+/+) and transgenic B-Tg(hIFNG/hIL26) mice (TG), and analyzed by ELISA with species-specific IFNγ ELISA kit. Human IFNγ was exclusively detectable in transgenic B-Tg(hIFNG/hIL26) mice but not in wild-type mice (B).

mRNA expression analysis

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Strain specific analysis of IL26 and IFNG mRNA expression in wild-type C57BL/6 mice and B-Tg(hIFNG/hIL26) mice by RT-PCR. Spleen (A) and Colon (B) RNA were isolated from wild-type C57BL/6 mice (+/+), B-Tg(hIFNG/hIL26) mice (TG) and DSS treated (3%, 6 d, drink) B-Tg(hIFNG/hIL26) mice (TG), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human IL26 and IFNG primers. Human IFNG mRNA was detectable in B-Tg(hIFNG/hIL26) mice spleen and colon. Human IL26 mRNA was detectable in B-Tg(hIFNG/hIL26) mice spleen and colon, and the level of IL26 mRNA in colon is up-regulated after DSS treated.