B-hIL4/hIL4RA/hIL13/hIL13RA1 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Cytokine Humanized Mice

Text

B-hIL4/hIL4RA/hIL13/hIL13RA1 mice

Strain Name

C57BL/6-Il4^{tm2(IL4)Bcgen} Il4ra^{tm1(IL4RA)Bcgen}

Il13^{tm1(IL13)Bcgen} Il13ra1^{tm1(IL13RA1)Bcgen} /Bcgen

Common Name

B-hIL4/hIL4RA/hIL13/hIL13RA1 mice

Background

C57BL/6

Catalog number

112812

Aliases

IL4: interleukin 4
IL4RA: interleukin 4 receptor, IL4R
Il13: interleukin 13
IL13RA1: CD213A1, CT19, IL-13Ra, NR4

Protein expression analysis in serum

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Strain specific IL4 expression analysis in homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice by ELISA. Serum were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1mice (H/H; H/H; H/H; H/H) stimulated with anti-mCD3Ɛ antibody in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse IL4 was detectable in wild-type C57BL/6 mice. Human IL4 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice, but not in wild-type mice.

Protein expression analysis in spleen

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Strain specific IL4RA expression analysis in homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice (H/H; H/H; H/H; H/H), and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse IL4RA was detectable in wild-type mice, Human IL4RA was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice, but not in wild-type mice.

Protein expression analysis

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Strain specific IL13 expression analysis in homozygous B-hIL4//hIL4RA/hIL13/hIL13RA1 mice by ELISA. CD4+ T cells were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice (H/H; H/H; H/H; H/H)，and then induction of CD4 + T cells differentiation into Th2 cells, supernatants from Th2 cells culture was analyzed by ELISA with species-specific IL13 ELISA kit. Mouse IL13 was detectable in wild-type mice. Human IL13 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice, but not in wild-type mice.

Functional analysis

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MHCⅡ expression analysis in wild-type and homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice by flow cytometry. Splenocytes from wild-type C57BL/6 mice and homozygous B-hIL4/hIL4RA/hIL13/hIL13RA1 mice were stimulated with mouse IL13 or human IL13 in vitro, resulting in increased MHCⅡ expression, respectively. Values are expressed as mean ± SD.