• ADGRG2 is a member of the G protein-coupled receptor family and is an epididymis specific transmembrane proteinGPR64 may be involved in a signal transduction pathway controlling epididymal function and male fertility. 
• The exons 3~29 of mouse Adgrg2 gene that encodes the full-length protein was replaced by human ADGRG2 exons 3~28 in B-hADGRG2 mice.
• ADGRG2 was detected in testis in both wild-type and homozygous B-hADGRG2 mice, as anti-ADGRG2 antibody was cross-reactive between human and mouse.

Strain specific analysis of ADGRG2 mRNA expression in wild-type C57BL/6 mice and B-hADGRG2 mice by RT-PCR. Testis RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hADGRG2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADGRG2 primers. Mouse ADGRG2 mRNA were detectable only in wild-type C57BL/6 mice. Human ADGRG2 mRNA was detectable only in homozygous B-hADGRG2 mice but not in wild-type mice.