B-hTPBG MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

Common name	B-hTPBG MC38	Catalog number	311068
Aliases	5T4	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Tpbg gene was replaced by human TPBG coding sequence in B-hTPBG MC38 cells. Human TPBG is highly expressed on the surface of B-hTPBG MC38 cells.

Targeting strategy

Gene targeting strategy for B-hTPBG MC38 cells. The exogenous promoter and human TPBG coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Tpbg gene, resulting in a non-functional transcript.

Protein expression analysis

TPBG expression analysis in B-hTPBG MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hTPBG MC38 cultures were stained with species-specific anti-TPBG antibody. Human TPBG was detected on the surface of B-hTPBG MC38 cells but not wild-type MC38 cells. The 2-C06 clone of B-hTPBG MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hTPBG MC38 cells. B-hTPBG MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hTPBG MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

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B-hTPBG MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5), and on 34 days post inoculation, tumor cells were harvested and assessed for human TPBG expression by flow cytometry. As shown, human TPBG was highly expressed on the surface of tumor cells. Therefore, B-hTPBG MC38 cells can be used for in vivo efficacy studies of novel TPBG therapeutics.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hTPBG MC38 cells. B-hTPBG MC38 cells (5x10⁵, 1x10⁶, 5x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into B-hCD3E mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hTPBG MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hTPBG MC38 cells. B-hTPBG MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hTPBG MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.