Common name
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B-hROR1 CT26.WT
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Catalog number
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322263
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Aliases
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NTRKR1, dJ537F10.1
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Disease
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Colon carcinoma
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Organism
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Mouse
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Strain
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BALB/c
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Tissue types
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Colon
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Tissue
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Colon
|
Description
The mouse
Ror1 gene was replaced by human
ROR1 coding sequence in B-hROR1 CT26.WT cells. Human ROR1 is highly expressed on the surface of B-hROR1 CT26.WT cells.
Application
B-hROR1 CT26.WT cells have the capability to establish tumors
in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-hROR1 CT26.WT cells. The exogenous promoter and human ROR1 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Ror1 gene, resulting in a non-functional transcript.
Protein expression analysis
ROR1 expression analysis in B-hROR1 CT26.WT cells by flow cytometry. Single cell suspensions from wild-type CT26.WT and B-hROR1 CT26.WT cultures were stained with species-specific anti-ROR1 antibody. Human ROR1 was detected on the surface of B-hROR1 CT26.WT cells but not wild-type CT26.WT cells.