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B-hPD-L2 MC38
Common name
B-hPD-L2 MC38 Catalog number    310703
Aliases PDCD1LG2, CD273,  PDCD1L2 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse PD-L2 gene was replaced by human PD-L2 coding sequence in B-hPD-L2 MC38 cells. Human PD-L2 is highly expressed on the surface of B-hPD-L2 MC38 cells.

Application


B-hPD-L2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.



Targeting strategy

Gene targeting strategy for B-hPD-L2 MC38 cells. The exogenous promoter and human PD-L2 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine PD-L2 gene, resulting in a non-functional transcript.

Protein expression analysis 

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PD-L2 expression analysis in B-hPD-L2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hPD-L2 MC38 cultures were stained with species-specific anti-PD-L2 antibody. Mouse PD-L2 was not detected on the surface of wild-type MC38 cells. Human PD-L2 was detected on the surface of B-hPD-L2 MC38 cells but not wild-type MC38 cells. The 1-C11 clone of B-hPD-L2 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hPD-L2 MC38 cells. B-hPD-L2 MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.


Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hPD-L2 MC38 cells. B-hPD-L2 MC38 cells (5x105, 1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hCD3E mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.