The mouse Pdl1 gene was replaced by human PD-L1 coding sequence in B-hPD-L1 MC38 cells. Human PD-L1 is highly expressed on the surface of B-hPD-L1 MC38 cells.
B-hPD-L1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
The exogenous promoter and human PD-L1 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Pdl1 gene, resulting in a non-functional transcript.
Protein expression analysis
PD-L1 expression analysis in B-hPD-L1 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hPD-L1 MC38 cultures were stained with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detected on the surface of wild-type MC38 cells. Human PD-L1 was detected on the surface of B-hPD-L1 MC38 cells but not wild-type MC38 cells. The D1 clone of B-hPD-L1 MC38 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes