top
Please input keywords
Order
*Country
日本
アメリカ
中国
オーストラリア
シンガポール
イギリス
フランス
ドイツ
スイス
イタリア
カナダ
韓国
オランダ
ベルギー
スウェーデン
その他
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hMMP-8 MC38
Common name
B-hMMP-8 MC38 Catalog number    322005
Aliases HNC; CLG1; MMP-8; PMNL-CL Disease  Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon
Description 

The mouse Mmp8 gene was replaced by human MMP-8 coding sequence in B-hMMP-8 MC38 cells. Human MMP-8 is highly expressed on the supernatant of B-hMMP-8 MC38 cells.

Application

B-hMMP-8 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hMMP-8 MC38 cells. The exogenous promoter and human MMP-8 coding sequence was inserted to replace part of murine exon 1-3. The insertion disrupts the endogenous murine Mmp8 gene, resulting in a non-functional transcript.

Protein expression analysis 

from clipboard


MMP-8 expression analysis in B-hMMP-8 MC38 cells by western blot. Human MMP-8 was detected in the supernatant of B-hMMP-8 MC38 cells but not wild-type MC38 cells. The 1-D01 and 1-G12 clones of B-hMMP-8 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

from clipboard

Subcutaneous homograft tumor growth of B-hMMP-8 MC38 cells. B-hMMP-8 MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 12-13-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMMP-8 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.


Tumor growth curve of individual mice

from clipboard


B-hMMP-8 MC38 tumor cells growth of individual mice. B-hMMP-8 MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 12-13-week-old, n=6). As shown in panel, B-hMMP-8 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.