Common name
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B-hLILRB4 MC38
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Catalog number
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310815
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Aliases
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ILT3, LIR5, CD85K, ILT-3, LIR-5
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Disease
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Colon carcinoma
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Organism
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Mouse
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Strain
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C57BL/6
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Tissue types
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Colon
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Tissue
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Colon
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Description
The human LILRB4 is expressed on the surface of B-hLILRB4 MC38 cells.
Application
B-hLILRB4 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-hLILRB4 MC38 cells. The exogenous promoter and human LILRB4 coding sequence was inserted to murine rosa26 site.
Protein expression analysis
LILRB4 expression analysis in B-hLILRB4 MC38 cells by flow cytometry. Single cell suspensions from B-hLILRB4 MC38 cultures were stained with species-specific anti-human LILRB4 antibody. Human LILRB4 was detected on the surface of B-hLILRB4 MC38 cells. The 5-C01 clone of B-hLILRB4 MC38 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5×long diameter × short diameter2. As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Protein expression analysis of tumor cells
B-hLILRB4 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=8), and on 34 days post inoculation, tumor cells were harvested and assessed for human LILRB4 expression by flow cytometry. As shown, human LILRB4 was highly expressed on the surface of tumor cells. Therefore, B-hLILRB4 MC38 cells can be used for in vivo efficacy studies of novel LILRB4 therapeutics.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells (5x105, 1x106, 5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hCD3E mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
