B-hHVEM MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

Common name	B-hHVEM MC38	Catalog number	310693
Aliases	TNFRSF14, CD270	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Hvem gene was replaced by human HVEM coding sequence in B-hHVEM MC38 cells. Human HVEM is highly expressed on the surface of B-hHVEM MC38 cells.

Targeting strategy

Gene targeting strategy for B-hHVEM MC38 cells. The exogenous promoter and human HVEM coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Hvem gene, resulting in a non-functional transcript.

Protein expression analysis

from clipboard

HVEM expression analysis in B-hHVEM MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hHVEM MC38 cultures were stained with species-specific anti-HVEM antibody. Mouse HVEM was detectable in wild-type MC38 cells. Human HVEM was detected on the surface of B-hHVEM MC38 cells but not wild-type MC38 cells. The 2-A5 clone of B-hHVEM MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHVEM MC38 cells. B-hHVEM MC38 cells (5x10⁵ and 1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hHVEM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.