B-hFAP MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

Common name	B-hFAP MC38	Catalog number	310825
Aliases	DPPIVA, FAPalpha, SIMP	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The CDS of human FAP was inserted to replace murine exons 7-9 in B-hFAP MC38 cells. Human FAP is highly expressed on the surface of B-hFAP MC38 cells.

Application

B-hFAP MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hFAP MC38 cells. The CDS of human FAP was inserted to replace murine exons 7-9. The insertion disrupts the endogenous murine FAP gene, resulting in a non-functional transcript.

Protein expression analysis

FAP expression analysis in B-hFAP MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hFAP MC38 cultures were stained with species-specific anti-FAP antibody. Human FAP was detected on the surface of B-hFAP MC38 cells but not wild-type MC38 cells. The 1-B04 clone of B-hFAP MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hFAP MC38 cells. B-hFAP MC38 cells (1x10⁶) and wild-type MC38 cells (1x10⁶) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hFAP MC38 cells. B-hFAP MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-hCD3E mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Protein expression analysis of tumor cells

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B-hFAP MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5), and on 32 days post inoculation, tumor cells were harvested and assessed for human FAP expression by flow cytometry. As shown, human FAP was highly expressed on the surface of tumor cells. Therefore, B-hFAP MC38 cells can be used for in vivo efficacy studies of novel FAP therapeutics.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hFAP MC38 cells. B-hFAP MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.