B-hCXCR2 MC38_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

Common name	B-hCXCR2 MC38	Catalog number	310824
Aliases	CD182, CMKAR2, IL8R2, IL8RA, IL8RB	Disease	Colon carcinoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Colon	Tissue	Colon

Description

The mouse Cxcr2 gene was replaced by human CXCR2 coding sequence in B-hCXCR2 MC38 cells. Human CXCR2 is highly expressed on the surface of B-hCXCR2 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hCXCR2 MC38 cells. The exogenous promoter and human CXCR2 coding sequence was inserted to replace part of murine exon 2 ~ 3’UTR. The insertion disrupts the endogenous murine Cxcr2 gene, resulting in a non-functional transcript.

Protein expression analysis

from clipboard

CXCR2 expression analysis in B-hCXCR2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCXCR2 MC38 cultures were stained with species-specific anti-CXCR2 antibody. Human CXCR2 was detected on the surface of B-hCXCR2 MC38 cells but not wild-type MC38 cells. The 6-F02 clone of B-hCXCR2 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hCXCR2 MC38 cells. B-hCXCR2 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCXCR2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.