top
Please input keywords
Order
*Country
日本
アメリカ
中国
オーストラリア
シンガポール
イギリス
フランス
ドイツ
スイス
イタリア
カナダ
韓国
オランダ
ベルギー
スウェーデン
その他
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hB7-H3 CT26.WT
Common name
B-hB7-H3 CT26.WT Catalog number    321829
Aliases CD276; B7H3; B7RP-2; 4Ig-B7-H3 Disease  Colon carcinoma
Organism
Mouse
Strain  BALB/c
Tissue types Colon Tissue  Colon

Description 


The mouse B7-h3 gene was replaced by human B7-H3 coding sequence in B-hB7-H3 CT26.WT cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 CT26.WT cells.


Application

B-hB7-H3 CT26.WT cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hB7-H3 CT26.WT cells. The chemic human and mouse B7-H3 coding sequence was inserted to replace part of murine exon 3 and exon 4. The insertion disrupts the endogenous murine B7-h3 gene, resulting in a non-functional transcript.

Protein expression analysis 


B7-H3 expression analysis in B-hB7-H3 CT26.WT cells by flow cytometry. Single cell suspensions from B-hB7-H3 CT26.WT cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 CT26.WT cells but not wild-type CT26.WT cells. The 3-H02 clone of B-hB7-H3 CT26.WT cells was used for in vivo experiments.


Tumor growth curve & Body weight changes

from clipboard

Subcutaneous homograft tumor growth of B-hB7-H3 CT26.WT cells. B-hB7-H3 CT26.WT cells and wild-type CT26.WT cells were subcutaneously implanted into BALB/c mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 CT26.WT cells were able to establish tumors in vivo and can be used for efficacy studies.


Protein expression analysis of tumor cells

from clipboard


B-hB7-H3 CT26.WT cells were subcutaneously transplanted into BALB/c mice (n=5). At the end of the experiment, tumor cells were harvested and assessed for human B7-H3 expression by flow cytometry. As shown, human B7-H3 was highly expressed on the surface of tumor cells. B-hB7-H3 CT26.WT cells can be used for in vivo efficacy studies of novel B7-H3 therapeutics.