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B-Tg(WT1 peptide-HLA-A2.1) MC38
Common name
B-Tg(WT1 peptide-HLA-A2.1) MC38 Catalog number    322369
Aliases HLA Disease  Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon

This cell line is for service only

Description 


The coding sequence of human B2M gene and the whole genomic sequence of human HLA-A2.1 gene were randomly inserted into the genome of B-Tg(WT1 peptide-HLA-A2.1) MC38. Human HLA-A2.1 is highly expressed on the surface of B-Tg(WT1 peptide-HLA-A2.1) MC38.

Application

B-Tg(WT1 peptide-HLA-A2.1) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-Tg(WT1 peptide-HLA-A2.1) MC38. The coding sequence of human B2M gene and the whole genomic sequence of human HLA-A2.1 gene were randomly inserted into the genome of B-Tg(WT1 peptide-HLA-A2.1) MC38. Human HLA-A2.1 is highly expressed on the surface of B-Tg(WT1 peptide-HLA-A2.1) MC38.

Protein expression analysis 

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HLA-A2.1 expression analysis in B-Tg(WT1 peptide-HLA-A2.1) MC38 by flow cytometry. Single cell suspensions from wild-type MC38 and B-Tg(WT1 peptide-HLA-A2.1) MC38 cultures were stained with species-specific anti-HLA-A2.1 antibody. Human HLA-A2.1 was detected on the surface of B-Tg(WT1 peptide-HLA-A2.1) MC38 cells but not wild-type MC38 cells. The 1-G03 clone of B-Tg(WT1 peptide-HLA-A2.1) MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-Tg(WT1 peptide-HLA-A2.1) MC38 cells. B-Tg(WT1 peptide-HLA-A2.1) MC38  cells (5x105) were subcutaneously implanted into B-HLA-A2.1 mice (female, 7-week-old, n=8). Tumor volume and body weight were measured three times a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(WT1 peptide-HLA-A2.1) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

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B-Tg(WT1 peptide-HLA-A2.1) MC38 tumor cells growth of individual mice. B-Tg(WT1 peptide-HLA-A2.1) MC38 (5x105) were subcutaneously implanted into B-HLA-A2.1 mice (female, 7-week-old, n=8). As shown in panel, B-Tg(WT1 peptide-HLA-A2.1) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.