Generation of gene editing mouse model and expression analysis

Phenotypic Analysis

Phenotypic analysis of B-luc-GFP Raji.

A. Fluorescence microscopy imaging showed green fluorescent expression in B-luc-GFP Raji;

B. The results showed that the fluorescence intensity of B-luc-GFP Raji cells was significantly higher than that of the control group. The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610).

Imaging analysis in vivo

Tumor growth and in vivo imaging of B-luc-GFP Raji cells. B-luc-GFP Raji cells (1x10⁶) were  injected by tail vein into B-NDG mice .Signal intensity and body weight were measured twice a week. (A) Imaging was performed on days 0, 4, 6, 8, 11, 15, and 18 , (B) Mice body weight (Mean ± SEM). B-luc-GFP Raji cells can be used for in vivo efficacy evaluation.

Human CD47Ab efficacy based on B-NDG mice

B-luc-GFP Raji cells were injected intraperitoneally into B-NDG and observed with a small animal imager. The animals were enrolled into the control group and the treatment group (n=6) when the tumor fluorescence intensity reached 1.5E6.

The results showed that different anti-human CD47 antibodies had different inhibitory effects on tumor growth at the same dose. A. Tumor fluorescence intensity ± SEM, B. Mean body weight ± SEM.