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B-Ncr1-EGFP-DTR-luciferase mice
Strain Name C57BL/6JNifdc-Ncr1tm2(EGFP-DTR-luc)Bcgen/Bcgen  Common Name  B-Ncr1-EGFP-DTR-luciferase mice
Background C57BL/6JNifdc Catalog number 112816
Aliases 
LY94, CD335, NKP46, NK-p46
NK cells depletion analysis in spleen

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Frequencies of NK cells and EGFP+ cells in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Ncr1 EGFP-DTR-luciferase mice (Mut/+) (n=3, 8-9-week-old) injected with PBS or DT (50 ng per g body weight) for four consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequencies of EGFP+ cells and NK cells in total CD45+ cells. The signal for NK cells(A) and EGFP+ cells(B) were decreased in heterozygous mice injected with DT. C. Percentages of NK cells and EGFP+ cells in total CD45+ populations. The frequencies of NK cells and EGFP+ cells were decreased in heterozygous mice after DT injection.

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Frequency of EGFP+ cells in NK cells from spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (+/+) and heterozygous B-Ncr1-EGFP-DTR-luciferase mice (Mut/+) (n=3, 8-9-week-old) injected with PBS or DT (50 ng per g body weight) for four consecutive days. Flow cytometry analysis of the splenocytes was performed to assess the frequency of EGFP in NK cells(A). The percentage of EGFP+ in NK cells(B) was decreased in heterozygous mice injected with DT.