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B-Nkp46-iCre mice
Strain Name

C57BL/6-Nkp46tm1(icre)Bcgen/Bcgen

Stock No.  110127
Common name

B-Nkp46-iCre mice

Gene symbol and name  Ncr1(natural cytotoxicity triggering receptor 1), NKp46; Ly94
Strain of origin

C57BL/6

Chromosome  7
Coat color Black Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
17086

Application

  • Function research of genes
  • This Ncr1iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of natural killer (NK) cells related signaling.

Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

The molecular is expressed on resting and activated NK cells


Gene editing strategy


from clipboard


An IRES-iCre sequence cassette was placed between the coding sequence of exon 7 and 3’UTR of the Nkp46 gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at least 300bp downstream of 3’UTR. This strain was maintained on a C57BL/6 genetic background.


Phenotype Analysis


from clipboard


NKp46 þ ILC3 cells from Washflox/flox and Washflox/floxNcr1-Cre mice were in situ hybridized with probes against Ahr promoter, followed by staining with antibodies against WASH and Arid1a (upper panel). White arrow head indicates Ahr promoters colocalized with Arid1a. Percentages of cells with Arid1a colocalized Ahr promoter were calculated (lower panel). At least 200 NKp46 þ ILC3 cells were counted. Scale bar, 5 mm.

(source: Xia P et al.,2017)

reference


1. Xia P, Liu J, Wang S, Ye B, Du Y, Xiong Z, Han ZG, Tong L, Fan Z. WASH maintains NKp46+ ILC3 cells by promoting AHR expression. Nat Commun. 2017 Jun 7;8:15685. [PubMed: 28589939]

2. Wang S, Xia P, Huang G, Zhu P, Liu J, Ye B, Du Y, Fan Z. FoxO1-mediated autophagy is required for NK cell development and innate immunity. Nat Commun. 2016 Mar 24;7:11023. [PubMed: 27010363]

3. Elboim M; Gazit R; Gur C; Ghadially H; Betser-Cohen G; Mandelboim O. 2010. Tumor immunoediting by NKp46. J Immunol 184(10):5637-44. [PubMed:20404273]

4. Ghadially H; Horani A; Glasner A; Elboim M; Gazit R; Shoseyov D; Mandelboim O. 2013. NKp46 regulates allergic responses. Eur J Immunol 43(11):3006-16. [PubMed: 23878025] 

5. Ghadially H; Ohana M; Elboim M; Gazit R; Gur C; Nagler A; Mandelboim O. 2014. NK Cell Receptor NKp46 Regulates Graft-versus-Host Disease. Cell Rep 7(6):1809-14. [PubMed: 24882008]

6. Glasner A; Ghadially H; Gur C; Stanietsky N; Tsukerman P; Enk J; Mandelboim O. 2012. Recognition and Prevention of Tumor Metastasis by the NK Receptor NKp46/NCR1. J Immunol 188(6):2509-15. [PubMed: 22308311]

7. Gur C; Porgador A; Elboim M; Gazit R; Mizrahi S; Stern-Ginossar N; Achdout H; Ghadially H; Dor Y; Nir T; Doviner V; Hershkovitz O; Mendelson M; Naparstek Y; Mandelboim O. 2010. The activating receptor NKp46 is essential for the development of type 1 diabetes. Nat Immunol 11(2):121-8. [PubMed: 20023661]

8. Chaushu S; Wilensky A; Gur C; Shapira L; Elboim M; Halftek G; Polak D; Achdout H; Bachrach G; Mandelboim O. 2012. Direct recognition of Fusobacterium nucleatum by the NK cell natural cytotoxicity receptor NKp46 aggravates periodontal disease. PLoS Pathog 8(3):e1002601. [PubMed:22457623]