B-hVLDLR mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

Strain Name	C57BL/6N-Vldlr^{tm1(VLDLR)Bcgen}/Bcgen	Common Name	B-hVLDLR mice
Background	C57BL/6N	Catalog number	111195
Aliases	VLDLR: CAMRQ1; CARMQ1; CHRMQ1; VLDL-R; VLDLRCH

Targeting strategy

Gene targeting strategy for B-hVLDLR mice.
The exons 2-17 of mouse Vldlr gene that encode the full-length protein were replaced by human VLDLR exons 2-17 in B-hVLDLR mice.

mRNA expression analysis

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Strain specific analysis of VLDLR mRNA expression in wild-type C57BL/6N mice and B-hVLDLR mice by RT-PCR. Testis and brain RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hVLDLR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human VLDLR primers. Mouse Vldlr mRNA was detectable only in wild-type C57BL/6N mice. Human VLDLR mRNA was detectable only in homozygous B-hVLDLR mice but not in wild-type mice.