Western blot analysis of TSPAN2 protein expression in homozygous B-hTSPAN2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTSPAN2 mice (H/H), and then analyzed by western blot with cross reactive anti-TSPAN2 antibody (Proteintech, 20463-1-AP). 40 μg total proteins were loaded for western blotting analysis. TSPAN2 was detected in heart, liver, lung, stomach, urinary bladder and ovary of both wild-type mice and homozygous mice.

Strain specific analysis of TSPAN2 mRNA expression in wild-type C57BL/6 mice and B-hTSPAN2 mice by RT-PCR. Lung and stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTSPAN2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TSPAN2 primers. Mouse Tspan2 mRNA was detectable only in wild-type C57BL/6 mice. Human TSPAN2 mRNA was detectable only in homozygous B-hTSPAN2 mice but not in wild-type mice.