| Strain Name |
C57BL/6-Igs2tm1(KIR3DL3/KIR2DL3/KIR2DL1/KIR2DL4/ KIR3DL1/KIR2DS4/KIR3DL2)Bcgen/Bcgen |
Common Name |
B-hKIR mice |
| Background | C57BL/6 | Catalog number |
114043 |
|
Related Genes |
KIR44, KIRC1, CD158Z, KIR3DL7, KIR2DL5B; p58, NKAT, GL183, NKAT2, CD158b; NKAT, NKAT1, p58.1, CD158A, KIR221; G9P, CD158D, KIR103, KIR-2DL4, KIR103AS; KIR, NKB1, NKAT3, NKB1B, NKAT-3, CD158E1; KKA3, KIR1D, NKAT8, CD158I, KIR412, NKAT-8; 3DL2, p140, NKAT4, CD158K, NKAT-4, NKAT4B, KIR-3DL2 |
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NCBI Gene ID |
115653, 3804, 3802, 3805, 3811, 3809, 3812 |
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Targeting strategy
Gene targeting strategy for B-hKIR mice. Bacterial artificial chromosome (BAC) clones for the human KIR3DL3, KIR2DL3, KIR2DL1, KIR2DL4, KIR3DL1, KIR2DS4 and KIR3DL2 genes, including the promoter, 5’UTR and 3’UTR are inserted into mouse Hipp11 (H11) locus in B-hKIR mice.
Protein expression analysis in spleen
Strain specific KIR-related protein expression analysis in wild-type C57BL/6JNifdc mice and humanized B-hKIR mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and B-hKIR mice(H/H) (male, 8-week-old, n=1). Protein expression was analyzed with anti-human KIR2DL1 antibody (Biolegend, 374904), anti-human KIR2DL3 antibody (Biolegend, 312612), anti-human KIR3DL1 antibody (Biolegend, 312708) and anti-human KIR3DL2 antibody (R&D System, FAB2878P-100) by flow cytometry, respectively. Human KIR2DL1, KIR2DL3, KIR3DL1 and KIR3DL2 were exclusively detectable in B-hKIR mice, but not in wild-type C57BL/6JNifdc mice.
Frequency of leukocyte subpopulations in the spleen
Frequency of leukocyte subpopulations in the spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6JNifdc mice and B-hKIR mice (H/H)(male, 8-week-old, n=3). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Frequencies of T cells, B cells, dendritic cells, monocytes, macrophages and neutrophils in B-hKIR mice were similar to those in C57BL/6JNifdc mice.
Frequency of NK populations in Spleen, Blood, Bone Marrow and Lung
Frequency of NK populations in Spleen, Blood, Bone Marrow and Lung by flow cytometry. Splenocytes, blood, bone marrow and lung were collected from wild-type C57BL/6JNifdc mice (+/+) and and homozygous B-hKIR mice (H/H) (male, 8-week-old, n=3; female, 8-week-old, n=3). No apparent difference was found between wild-type C57BL/6JNifdc mice (+/+) and B-hKIR mice.
