B-hTEK/hTIE1 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Other humanized mice

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B-hTEK/hTIE1 mice

Strain Name	C57BL/6-Tek^{tm1(TEK)Bcgen} Tie1^{tm1(TIE1)Bcgen}/Bcgen	Common Name	B-hTEK/hTIE1 mice
Background	C57BL/6	Catalog number	112513
Aliases	CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1; JTK14, LMPHM11, TIE

Protein expression analysis-TEK

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Strain specific TEK expression analysis in homozygous B-hTEK/hTIE1 mice by flow cytometry. Lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTEK/hTIE1 mice (H/H, H/H), and analyzed by flow cytometry with anti-TEK antibody. mTEK was detectable in wild-type, hTEK was detectable in wild-type and homozygous B-hTEK/hTIE1 mice, as the antibody is cross-recognize both human and mouse TEK.

Protein expression analysis-TIE1

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Strain specific TIE1 expression analysis in homozygous B-hTEK/hTIE1 mice by flow cytometry. Lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTEK/hTIE1 mice (H/H, H/H), and analyzed by flow cytometry with anti-TIE1 antibody. hTIE1 was detectable in wild-type and homozygous B-hTEK/hTIE1 mice, as the antibody is cross-recognize both mouse and human TIE1.

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Western blot analysis of TIE1 protein expression in homozygous B-hTEK/hTIE1 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hTEK/hTIE1 mice (H/H), and then analyzed by western blot with anti-TIE1 antibody. 40 μg total proteins were loaded for western blotting analysis. TIE1 was detected in lung, kidney, spleen and heart in wild-type mice and homozygous B-hTEK/hTIE1 mice, as the antibody is cross-recognize both human and mouse TIE1.

mRNA expression analysis

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Strain specific analysis of TEK mRNA expression in wild-type C57BL/6 mice and B-hTEK/hTIE1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTEK/hTIE1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TEK primers. Mouse Tek mRNA was detectable only in wild-type C57BL/6 mice. Human TEK mRNA was detectable only in homozygous B-hTEK/hTIE1 mice but not in wild-type mice.

Strain specific analysis of TIE1 mRNA expression in wild-type C57BL/6 mice and B-hTEK/hTIE1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTEK/hTIE1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TIE1 primers. Mouse Tie1 mRNA was detectable only in wild-type C57BL/6 mice. Human TIE1 mRNA was detectable only in homozygous B-hTEK/hTIE1 mice but not in wild-type mice.