B-hCD16A mice(C)_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Immune-checkpoint Humanized Mice

Text

B-hCD16A mice(C)

Strain Name	BALB/c-Fcgr4^{tm2(FCGR3A)Bcgen}/Bcgen	Common Name	B-hCD16A mice(C)
Background	BALB/cCrSlcNifdc	Catalog number	111885
Related Genes	FCGR3A (Fc fragment of IgG receptor IIIa), CD16A

Protein expression analysis in blood and spleen-NK cells

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Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was not detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).

Protein expression analysis in blood and spleen-Monocytes

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Protein expression analysis in blood and spleen-Macrophages

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Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was detectable in wild-type mice. Human CD16A was weakly detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).

Protein expression analysis in blood and spleen-Granulocytes

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Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).

Protein expression analysis in blood and spleen-PEMs

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Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C).

Analysis of leukocytes cell subpopulation in spleen

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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female BALB/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in spleen

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Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in lymph node

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Analysis of LNs leukocyte subpopulations by FACS. LNs were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in lymph node

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Analysis of LNs T cell subpopulations by FACS. LNs were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in blood

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Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in blood

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Analysis of blood T cell subpopulations by FACS. Blood were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.