B-h4-1BB/hHER2 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Other humanized mice

Text

B-h4-1BB/hHER2 mice

Strain Name

C57BL/6-Tnfrsf9^{tm1(TNFRSF9)Bcgen} Erbb2^{tm1(ERBB2)Bcgen}/Bcgen

Common Name

B-h4-1BB/hHER2 mice

Background

C57BL/6

Catalog number

111869

Related Genes

TNFRSF9 (tumor necrosis factor receptor superfamily, member 9);

ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, VSCN2

mRNA expression analysis

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Strain specific analysis of 4-1BB gene expression in wild type (WT) mice and B-h4-1BB/hHER2 mice by RT-PCR. Mouse Tnfrsf9 mRNA was detectable only in spleen of WT mice (+/+). Human TNFRSF9 mRNA was detectable only in homozygous B-h4-1BB/hHER2 mice (H/H;H/H) but not in WT mice (+/+).

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Strain specific analysis of HER2 gene expression in wild type (WT) mice and B-h4-1BB/hHER2 mice by RT-PCR. Mouse Erbb2 mRNA was detectable only in liver of WT mice (+/+). Human ERBB2 mRNA was detectable only in homozygous B-h4-1BB/hHER2 mice (H/H;H/H) but not in WT mice (+/+).

Protein expression analysis

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Strain specific 4-1BB expression analysis in homozygous B-h4-1BB/hHER2 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-h4-1BB/hHER2 mice (H/H;H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-4-1BB antibody. Mouse 4-1BB was detectable in WT mice (+/+). Human 4-1BB was exclusively detectable in homozygous B-h4-1BB/hHER2 mice (H/H;H/H) but not in WT mice (+/+).

IHC analysis of HER2 expression

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Immunohistochemical (IHC) analysis of HER2 expression in homozygous B-h4-1BB/hHER2 mice. The liver and mammary gland were collected from WT mice and homozygous B-h4-1BB/hHER2 mice and analyzed by IHC with anti-HER2 antibody. HER2 was detectable in WT mice and homozygous B-h4-1BB/hHER2 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER2 staining (brown).