B-hPD-1/hPD-L1/hIL1B mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Cytokine Humanized Mice

Text

B-hPD-1/hPD-L1/hIL1B mice

Strain Name	C57BL/6-Pdcd1^{tm1(PDCD1)Bcgen} Cd274^{tm1(CD274)Bcgen} Il1b^{tm1(IL1B)Bcgen}/Bcgen	Common Name	B-hPD-1/hPD-L1/hIL1B mice
Background	C57BL/6	Catalog number	131619
Related Genes	PD-1(CD279, PDCD1, PD1, SLEB2, hPD-1, hPD-l, hSLE1) ；PD-L1 (B7-H, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1, hPD-L1) IL1B（IL-1; IL1F2; IL1beta; IL1-BETA）

Protein expression analysis

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Strain specific PD-1 and PD-L1 expression analysis in wild-type C57BL/6 mice and homozygous B-hPD-1/hPD-L1/hIL1B mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hIL1B mice (H/H) after stimulated with or without anti-CD3ε in vivo. Mouse PD-1 and PD-L1 were detectable in wild-type C57BL/6 mice. Human PD-1 and PD-L1 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL1B mice but not in wild-type mice.

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Strain specific IL1B expression analysis in homozygous B-hPD-1/hPD-L1/hIL1B mice by ELISA. Bone marrow cells were collected from C57BL/6 (+/+) and homozygous B-hPD-1/hPD-L1/hIL1B mice (H/H) and stimulated with LPS for 24 hours in vitro. The supernatant was analyzed with species-specific IL1B ELISA kit. Mouse IL1B was detectable in C57BL/6 mice. Human IL1B was exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL1B mice but not C57BL/6 mice. ND, not detectable.