|Strain Name||C57BL/6-Pdcd1tm1(PDCD1)Cd274tm1(CD274)Il1btm1(IL1B)/Bcgen||Common Name||B-hPD-1/hPD-L1/hIL1B mice|
PD-1(CD279, PDCD1, PD1, SLEB2, hPD-1, hPD-l, hSLE1) ；PD-L1 (B7-H, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1, hPD-L1)
IL1B（IL-1; IL1F2; IL1beta; IL1-BETA）
Protein expression analysis
Strain specific PD-1 and PD-L1 expression analysis in wild-type C57BL/6 mice and homozygous B-hPD-1/hPD-L1/hIL1B mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hIL1B mice (H/H) after stimulated with or without anti-CD3ε in vivo. Mouse PD-1 and PD-L1 were detectable in wild-type C57BL/6 mice. Human PD-1 and PD-L1 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL1B mice but not in wild-type mice.