Strain specific IgE expression analysis in homozygous B-hIgE/hFCER1A mice by flow cytometry. Basophilic granulocytes of the bone marrow were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIgE/hFCER1A mice (H/H;H/H) stimulated with LPS in vivo, and analyzed by flow cytometry with species-specific anti-IgE antibody. Mouse IgE was detectable in WT mice (+/+). Human IgE was exclusively detectable in homozygous B-hIgE/hFCER1A mice (H/H;H/H) but not in WT mice (+/+).

Strain specific FCER1A expression analysis in homozygous B-hIgE/hFCER1A mice by flow cytometry. Basophilic granulocytes of the bone marrow were collected from wild type (WT) mice (+/+) and homozygous B-hIgE/hFCER1A mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-FCER1A antibody. Mouse FCER1A was detectable in WT mice (+/+). Human FCER1A was exclusively detectable in homozygous B-hIgE/hFCER1A mice (H/H;H/H) but not in WT mice (+/+).