B-hIL4/hIL4RA/hIL13 mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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動物・細胞モデル

Humanized Mouse Models

Cytokine Humanized Mice

Text

B-hIL4/hIL4RA/hIL13 mice

Strain Name	C57BL/6-Il4^{tm2(IL4)Bcgen} Il4ra^{tm1(IL4RA)Bcgen} Il13^{tm1(IL13)Bcgen} Il13ra1^{tm1(IL13RA1)Bcgen} /Bcgen	Common Name	B-hIL4/hIL4RA/hIL13 mice
Background	C57BL/6	Catalog number	131037
Related Genes	IL4: interleukin 4; IL4RA: interleukin 4 receptor, IL4R; Il13:interleukin 13
NCBI Gene ID	16189,16190,16163

mRNA expression analysis

Strain specific analysis of IL4, IL4RA and IL13 gene expression in wild-type and B-hIL4/hIL4RA/hIL13 mice by RT-PCR. Mouse Il4, Il4ra and Il13 mRNA was detectable in thymocytes of wild-type mice (+/+) but not in homozygous B-hIL4/hIL4RA/hIL13 mice (H/H). Human IL4, IL4RA and IL13 mRNA was detectable only in homozygous B-hIL4/hIL4RA/hIL13 mice (H/H) but not in wild-type mice.

Protein expression analysis

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Strain specific IL4 expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by ELISA.

Serum were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse IL4 was detectable in wild-type mice. Human IL4 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.

Strain specific IL13 expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by ELISA.

CD4+ T cells were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H), and then induction of CD4 + T cells differentiation into Th2 cells, supernatants from Th2 cells culture was analyzed by ELISA with species-specific IL13 ELISA kit. Mouse IL13 was detectable in wild-type mice. Human IL13 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.

Protein expression analysis

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Strain specific IL4RA expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by flow cytometry. Splenocytes were collected from wild-type mice and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H), and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse IL4RA was detectable in wild-type mice. Human IL4RA was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.