Strain specific analysis of IL36R gene expression in wild type (WT) mice, B-hIL36R mice and B-hIL36R/hIL1RACP mice by RT-PCR. Human IL36R mRNA was detectable only in lung of homozygous B-hIL36R mice (H/H) and homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.

Strain specific IL1RACP expression analysis in homozygous B-hIL36R/hIL1RACP mice by flow cytometry. Monocytes, macrophages and neutrophils were collected from wild type (WT) mice (+/+) and homozygous B-hIL36R/hIL1RACP mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-hIL1RACP antibody. Human IL1RACP was exclusively detectable in homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+).