Strain specific analysis of IGF1R gene expression in wild-type (WT) mice (+/+) and homozygous B-hIGF1R mice (H/H) by RT-PCR. Mouse Igf1r mRNA was detectable only in brain of WT mice (+/+). Human IGF1R mRNA was detectable only in homozygous B-hIGF1R mice (H/H) but not in WT mice (+/+).

Strain specific IGF1R expression analysis in homozygous B-hIGF1R mice by flow cytometry. Kidney was collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by flow cytometry with anti-IGF1R antibody.IGF1R was detectable in wild-type mice and homozygous B-hIGF1R mice, as the antibody is crossly reactive with IGF1R in human and mice. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.

Strain specific IGF1R expression analysis in homozygous B-hIGF1R mice by flow cytometry. Brain was collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by flow cytometry with anti-IGF1R antibody.IGF1R was detectable in wild-type mice and homozygous B-hIGF1R mice, as the antibody is crossly reactive with IGF1R in human and mice. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.

Strain specific IGF1R expression analysis in homozygous B-hIGF1R mice by flow cytometry. Lung was collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by flow cytometry with anti-IGF1R antibody.IGF1R was detectable in wild-type mice and homozygous B-hIGF1R mice, as the antibody is crossly reactive with IGF1R in human and mice. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.

Immunofluorescence analysis of isolated brain microvessels from the wild-type mice and homozygous B-hIGF1R mice. Brain microvessels were collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by immunofluorescence with anti-hIGF1R antibody. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.