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B-hGPR40 mice
Strain Name
C57BL/6N-Frar1tm1(FFAR1)Bcgen/Bcgen
Common Name  B-hGPR40 mice
Background C57BL/6N Catalog number 111595
Aliases 
GPR40: FFA1R, GPCR40
Protein expression analysis

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Western blot analysis of FFAR1 protein expression in homozygous B-hFFAR1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFFAR1 mice (H/H), and then analyzed by western blot with species-specific anti-FFAR1 antibody. 20 μg total proteins were loaded for western blotting analysis. hFFAR1 was detected in pancreas from homozygous mice but not in liver,kidney and stomach.

mRNA expression analysis

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Strain specific analysis of FFAR1 mRNA expression in wild-type C57BL/6 mice and B-hFFAR1 mice by RT-PCR. BM RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hFFAR1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FFAR1 primers. Mouse Ffar1 mRNA was detectable only in wild-type C57BL/6 mice. Human FFAR1 mRNA was detectable only in homozygous B-hFFAR1 mice but not in wild-type mice.