B-hGLP1R/hGCGR mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

Strain Name	C57BL/6-Glp1r^tm1(GLP1R)Gcgr^tm1(GCGR)/Bcgen	Common Name	B-hGLP1R/hGCGR mice
Background	C57BL/6	Catalog number	121837
Aliases	GLP-1; GLP-1R; GLP-1-R GGR; GL-R

mRNA expression analysis

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Strain specific analysis of GLP1R gene expression in wild-type mice and B-hGLP1R/hGCGR mice by RT-PCR. Mouse Glp1r mRNA was detectable in lung and liver of wild-type mice (+/+). Human GLP1R mRNA was detectable in lung and liver of homozygous B-hGLP1R/hGCGR mice.

Protein expression analysis

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GLP1R expression analysis in wild-type mice and B-hGLP1R/hGCGR mice by western blotting. GLP1R protein was detectable in the lung, brain and liver of wild-type mice (+/+) and homozygous B-hGLP1R/hGCGR mice (H/H). As for the antibody used in the experiments have cross reaction between human and mouse, mRNA data should be considered. For mouse Glp1r mRNA was not expressed anymore, it’s predicted that the expressed protein in B-hGLP1R/hGCGR mice was human GLP1R.

mRNA expression analysis

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Strain specific analysis of GCGR gene expression in wild-type mice and B-hGLP1R/hGCGR mice by RT-PCR. Mouse Gcgr mRNA was detectable in kidney and liver of wild-type mice (+/+). Human GCGR mRNA was detectable in kidney and liver of homozygous B-hGLP1R/hGCGR mice (H/H).

Protein expression analysis

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GCGR expression analysis in wild-type mice and B-hGLP1R/hGCGR mice by western blotting. GCGR protein was detectable in the kidney and liver of wild-type mice (+/+) and homozygous B-hGLP1R/hGCGR mice (H/H). As for the antibody used in the experiments have cross reaction between human and mouse, mRNA data should be considered. For mouse Gcgr mRNA was not expressed anymore, it’s predicted that the expressed protein in B-hGLP1R/hGCGR mice was human GCGR.