mRNA and protein expression analysis

Strain specific analysis of CALCRL gene expression in B-CALCRL mice by RT-PCR and western blot. A. Mouse Calcrl mRNA was detectable in lung of wild-type (+/+) mice. Human CALCRL mRNA was detectable only in homozygous B-hCALCRL mice (H/H) but not in wild-type mice. B. Human CALCRL protein was detectable in lung of homozygous B-hCALCRL mice (H/H) and wild type mice,but not in cerebellum. This anti-human CALCRL antibody cross reacts with mouse Calcrl. Taken together, the results suggest that B-hCALCRL mice successfully express human CALCRL.