B-Luc ID8_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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B-Luc ID8

Common name	B-Luc ID8	Catalog number	322223
Aliases	NA	Disease	Ovarian cancer
Organism	Mouse	Strain	C57BL/6
Tissue types	Ovary	Tissue	Ovary

Description

This B-Luc ID8 cell line expresses firefly luciferase as a marker of ID8 cells. Fluorescence could be detected in B-Luc ID8 cells.

Application

B-Luc ID8 cell line can be used to evaluate anti-cancer drugs in ovarian cancer in vivo. It can be applied subcutaneously and orthotopically to ovaries in mice

Targeting strategy

Gene targeting strategy for B-Luc ID8 cells. The CMV promoter and luciferase were inserted into the targeting vector used in the lentivirus system.

In vitro luciferase activity of B-Luc ID8 cells

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Luminescence signal intensity of B-Luc ID8 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc ID8 cells have a strong luminescence signal. The mix clone of B-Luc ID8 cells can be used in vivo experiments.

Quantitative imaging data & body weight changes

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B-Luc ID8 cells (5x10⁵, 1x10⁶) were injected into the ovary of wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc ID8 cells can be used for in vivo efficacy evaluation.

Note:

The fluorescence signal on the 14th day decreased because the mouse hair was not removed completely.

Imaging analysis in vivo

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Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-Luc ID8 cells. B-Luc ID8 cells (1x10⁶) and wild-type ID8 cells (1x10⁶) were subcutaneously implanted into C57BL/6 mice (female, 7-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-Luc ID8 cells. B-Luc ID8 cells (1x10⁶) and wild-type ID8 cells (1x10⁶) were subcutaneously implanted into C57BL/6 mice (female, 5-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Quantitative imaging data & body weight changes

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Tumor growth and in vivo imaging of B-Luc ID8 cells. B-Luc ID8 cells (1x10⁶) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc ID8 cells can be used for in vivo efficacy evaluation.