B-luc B16-F10_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen,drug development,antibody discovery

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B-luc B16-F10

Common name	B-luc B16-F10	Catalog number	310680
Aliases	NA	Disease	Melanoma
Organism	Mouse	Strain	C57BL/6
Tissue types	Skin	Tissue	Skin

Description

This B-luc B16-F10 cell line expresses firefly luciferase as a marker of B16-F10 cells. Luminescence can be observed B-luc B16-F10 cells.

Application

B-luc B16-F10 cell line can be used to evaluate anti-cancer drugs in melanoma in vivo.

Gene targeting strategy

Gene targeting strategy for B-luc B16-F10 . The luciferase cDNA sequence with CAG promoter was inserted in the ROSA26 exon1 of wild-type B16-F10.

In vitro luciferase activity of B-luc B16-F10 cells

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Luminescence signal intensity of B-luc B16-F10 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-luc B16-F10 cells have a strong luminescence signal. The C5 clone of B-luc B16-F10 cells can be used in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-luc B16-F10 cells. B-luc B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-luc B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Quantitative imaging data & body weight changes

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Tumor growth and in vivo imaging of B-luc B16-F10 cells. B-luc B16-F10 cells (1x10⁵ and 2x10⁵) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-luc B16-F10 cells can be used for in vivo efficacy evaluation.