top
Please input keywords
Order
*Country
日本
アメリカ
中国
オーストラリア
シンガポール
イギリス
フランス
ドイツ
スイス
イタリア
カナダ
韓国
オランダ
ベルギー
スウェーデン
その他
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-Tagln-iCre mice
Strain Name

C57BL/6-Taglntm1(icre)Bcgen/Bcgen

Stock No.  110146
Common name

B-Tagln-iCre mice

Gene symbol and name  Tagln, transgelin
Strain of origin

C57BL/6N

Chromosome  9
Coat color Black Dietary information Growth and reproduction diet for experimental mice
NCBI Gene ID
21345

Application

  • Function research of genes
  • Function research of smooth muscle cell

Expression


Expressed gene

iCre, improved cre recombinase, bacteriophage P1


Site of expression

smooth muscle cell


Gene editing strategy


from clipboard


ICRE, WPRE and PolyA cassette were inserted into the first intron of mouse endogenous transgelin gene to express iCre recombinase regulated by the Tagln promoter of mouse endogenous gene, and the PolyA element knocked out the Tagln gene in mice.The correct genotype mice were obtained by microinjection of mouse ES cells. This design is based on transcript-201 (ENSMUST00000034590.3, NM_011526 ) This gene encodes a smooth muscle cell-specific cytoskeletal protein. The encoded protein is structurally similar to calponin, an actin-binding protein. In mouse models of atherosclerosis the gene product may be involved in plaque cell and atherosclerotic lesion formation during atherogenesis.


Phenotype analysis

from clipboardfrom clipboard

from clipboardfrom clipboard



The frozen sections of the stomach (a), aorta (b), jejunum (c) and bladder (d) of mice were detected by IF, respectively, and the results showed that tdTomato specific expression in Tagln-iCre (Mut /+);CAG-tdTomato (Mut / +) genotypes of double positive mice in alpha SMA markers of smooth muscle cells (with SMA protein markers of smooth muscle cells, the vast majority of the proportion of cells containing tdTomato red fluorescent protein signals, but it is not 100%, analyze the possible reasons may be the efficiency of the recombinant enzyme wasn't 100%), can attest, iCre recombinant enzyme specificity expressed in smooth muscle cells, other tissue cells tdTomato red fluorescent protein signal wasn’t detected. The mice in the negative control group also did not express tdTomato red fluorescent protein.


References


1.Yang M, Jiang H, Li L. Sm22α transcription occurs at the early onset of the cardiovascular system and the intron 1 is dispensable for its transcription in smooth muscle cells during mouse development. Int J Physiol Pathophysiol Pharmacol. 2010;2(1):12-9. Epub 2009 Nov 22. [PMID: 20428474]; [PMCID: PMC2860299]

2.Zhang JC, Kim S, Helmke BP, Yu WW, Du KL, Lu MM, Strobeck M, Yu Q, Parmacek MS. Analysis of SM22alpha-deficient mice reveals unanticipated insights into smooth muscle cell differentiation and function. Mol Cell Biol. 2001 Feb;21(4):1336-44. [PMID: 11158319]; [PMCID: PMC99586]

3.Lepore JJ, Cheng L, Min Lu M, Mericko PA, Morrisey EE, Parmacek MS. High-efficiency somatic mutagenesis in smooth muscle cells and cardiac myocytes in SM22alpha-Cre transgenic mice. Genesis. 2005 Apr;41(4):179-84. [PMID: 15789423]

4.Regan CP, Manabe I, Owens GK. Development of a smooth muscle-targeted cre recombinase mouse reveals novel insights regarding smooth muscle myosin heavy chain promoter regulation. Circ Res. 2000 Sep 1;87(5):363-9. [PMID: 10969033]

5.Yan J, Zhang L, Sultana N, Park DS, Shekhar A, Bu L, Hu J, Razzaque S, Cai CL. A Murine Myh6MerCreMer Knock-In Allele Specifically Mediates Temporal Genetic Deletion in Cardiomyocytes after Tamoxifen Induction. PLoS One. 2015 Jul 23;10(7):e0133472. [PMID: 26204265]; [PMCID: PMC4512710]