B-NDG Hc mice
C5, C5a, He, Hfib, Hfib2
Measurement of C5 in Serum
C5 expression analysis in homozygous B-NDG Hc mice by ELISA. Serum were collected from C57BL/6, B-NDG mice and homozygote B-NDG Hc mice, and analyzed by ELISA with C5 ELISA kit. Mouse C5 was detectable in C57BL/6 mice and B-NDG Hc mice. but not B-NDG mice.
Measurement of total complement hemolytic activity in Serum
Hemolytic capacity analysis in homozygous B-NDG Hc mice. Representative image demonstrating level of lysis of antibody-coated SRBC following incubation with diluted serum from human, C57BL6, B-NDG, or B-NDG Hc mice. Serum from human were used as positive complement control. Compared with B-NDG, the hemolytic ability of B-NDG Hc was significantly enhanced. The order of hemolytic capacity: B-NDG Hc ≥ C57BL/6 > B-NDG.