HLA-A2.1 expression analysis in homozygous B-NDG HLA-A2.1 mice by flow cytometry. Splenocytes were collected from B-NDG mice (+/+) and homozygous B-NDG HLA-A2.1 mice (H/H), and analyzed by flow cytometry with strain specific antibodies. Mouse H-2Kb/H-2Db was detectable in B-NDG mice but not in B-NDG HLA-A2.1 mice. Human HLA-A2.1 was exclusively detectable in homozygous B-NDG HLA-A2.1 mice but not in B-NDG mice.

Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female B-NDG mice and B-NDG HLA-A2.1 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of dendritic cells, granulocytes, monocytes and macrophages in homozygous B-NDG HLA-A2.1 mice were similar to those in the B-NDG mice, demonstrating that B2M and HLA-A2.1 humanized does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.

Analysis of bone marrow leukocyte subpopulations by FACS. Bone marrow was isolated from female B-NDG mice and B-NDG HLA-A2.1 mice (n=3, 6-week-old). Flow cytometry analysis was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of dendritic cells, granulocytes, monocytes and macrophages in homozygous B-NDG HLA-A2.1 mice were similar to those in the B-NDG mice, demonstrating that B2M and HLA-A2.1 humanized does not change the overall development, differentiation or distribution of these cell types in bone marrow. Values are expressed as mean ± SEM.

Analysis of blood leukocyte subpopulations by FACS. Blood was isolated from female B-NDG mice and B-NDG HLA-A2.1 mice (n=3, 6-week-old). Flow cytometry analysis was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of dendritic cells, granulocytes, monocytes and macrophages in homozygous B-NDG HLA-A2.1 mice were similar to those in the B-NDG mice, demonstrating that B2M and HLA-A2.1 humanized does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.