B16-F10-OVA cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Protein expression analysis
OVA expression analysis in B16-F10-OVA cells by flow cytometry. Single cell suspensions from wild-type B16-F10 and B16-F10-OVA cultures were stained with species-specific anti-OVA antibody. OVA was detected on the surface of B16-F10-OVA cells but not wild-type B16-F10 cells. The clones 1-G12 and 1-G04 of B16-F10-OVA cells were used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B16-F10-OVA cells. B16-F10-OVA cells (2x105) and wild-type B16-F10 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B16-F10-OVA cells were able to establish tumors in vivo and can be used for efficacy studies.