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B-hSIGLEC15 MC38
Common name
B-hSIGLEC15 MC38 Catalog number     311049
Aliases CD33L3, HsT1361, SIGLEC-15 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description


The mouse Siglec15 gene was replaced by human SIGLEC15 coding sequence in B-hSIGLEC15 MC38 cells. Human SIGLEC15 is highly expressed on the surface of B-hSIGLEC15 MC38 cells.


Targeting strategy


Gene targeting strategy for B-hSIGLEC15 MC38 cells. The exogenous promoter and human SIGLEC15 coding sequence was inserted to replace part of murine exon 3 ~ 3’UTR. The insertion disrupts the endogenous murine Siglec15 gene, resulting in a non-functional transcript.

Protein expression analysis


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SIGLEC15 expression analysis in B-hSIGLEC15 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hSIGLEC15 MC38 cultures were stained with species-specific anti-SIGLEC15 antibody. Human SIGLEC15 was detected on the surface of B-hSIGLEC15 MC38 cells but not wild-type MC38 cells. The 3-D10 clone of B-hSIGLEC15 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hSIGLEC15 MC38 cells. B-hSIGLEC15 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hSIGLEC15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Recommendations for inoculation in other mouse strains: 
Cell inoculation amount is recommended to be tried between 5x105-1x107
Inoculated cells can be tried to be suspended with DMEM stock solution.