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B-hPSMA MC38
Common name
B-hPSMA MC38 Catalog number     310840
Aliases  / Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description


The human PSMA coding sequence was inserted to mouse PSMA gene locus site. Human PSMA is highly expressed on the surface of B-hPSMA MC38 cells.

Application

B-hPSMA MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis 



PSMA expression analysis in B-hPSMA MC38 cells by flow cytometry. Single cell suspensions from B-hPSMA MC38 cultures were stained with species-specific anti-PSMA antibody. Human PSMA was detected on the surface of B-hPSMA MC38 cells but not wild-type MC38 cells. The 2-G05 clone of B-hPSMA MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hPSMA MC38 cells. B-hPSMA MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hPSMA mice (male, 8-week-old, n=4 or 5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hPSMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.