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B-hPD-L1 low MC38 plus
Common name
B-hPD-L1 low MC38 plus Catalog number     321853
Aliases B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The chimeric CDS composed of mouse signal peptide, human IgV region, mouse extracellular region except IgV domain, moue transmembrane region and mouse intracellular region of PD-L1 gene was inserted to replace the IgV domain of mouse Pdl1 gene. Human PD-L1 is highly expressed on the surface of B-hPD-L1 low MC38 plus cells.

Application

B-hPD-L1 low MC38 plus cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis 


PD-L1 expression analysis in B-hPD-L1 low MC38 plus cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hPD-L1 low MC38 plus cultures were stained with species-specific anti-PD-L1 antibody. Human PD-L1 was detected on the surface of B-hPD-L1 low MC38 plus cells but not wild-type MC38 cells. The 2-D10 clone of B-hPD-L1 low MC38 plus cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hPD-L1 low MC38 plus cells. B-hPD-L1 low MC38 plus cells (1x106), B-hPD-L1 low MC38 plus cells (3x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L1 low MC38 plus cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.